Abstract
In order to clarify the meaning and mechanism of complement changes in patients with chronic glomerulonephritis requiring, chronic hemodialysis complement fragments C4d, iC3b & Bb, strict indicators of complement activation, were measured by ELISA using monoclonal antibody (Fuji, Rebio). C3, C4, and CH50 were also detected. At the same time measurement of circulating immune complexes (CIC) was carried out by ELISA (Cytotec CIC Kit: C1q method) to detect complement activating factor.
The results were as follows: complement fragments-C4d, iC3b & Bb-and CIC in chronic hemodialysis (HD) patients showed significantly higher levels than those in normal subjects. Furthermore, the levels of C4d and iC3b in HD patients with high CIC were significantly increased over those with normal CIC.
In conclusion; 1. In addition to alternative pathway, classical pathway with which CIC were involved was also activated in HD patients with glomerulonephritis. 2. No correlation between complement fragments and the duration of hemodialysis was seen.
These facts are very interesting considering the clinical symptoms and various complications of HD patients.
