Evaluation of creatinine determination by enzymatic assay and the Jaffe reaction method in peritoneal equilibration test

Abstract

We measured creatinine in peritoneal dialysate by enzymatic assay (EA) and the Jaffe reaction method (J), to perform peritoneal equilibration tests (PET) in children. Average D2/P ratios measured by J (corrected by glucose), and EA of D2/P were 0.494 and 0.478 (p<0.01), respectively, and those of D4/P were 0.788 and 0.722 (p<0.01), respectively. EA is more accurate and useful for measuring creatinine in peritoneal dialysate since this method is not influenced by the glucose concentration of the dialysate.