Abstract
Infrared absorption spectra of several kinds of HD membranes before and after HD treatment were obtained by Fournier transform infrared (FTIR) spectroscopy fitted with an attenuated total reflection (ATR) attachment with Ge reflection elements in order to analyze the external membrane surface.
For cases of cuproammonium rayon membrane and modified cellulose membranes such as DEAE and Vit. E-coated cellulose membrane, two new bands assigned to C=O stretching vibration mode (amide I band) and N-H deforming vibration mode (amide II band) were observed after use, indicating adsorption of proteins on the membrane surface. For PEG-coated modified cellulose membrane, there was no change in the spectrum after use. For cellulose triacetate membrane, C=O strecthing vibration band assigned to acetyl groups of the membrane shifted to a lower wavenumber after use, indicating chemical adsorption of proteins on the acetyl groups of membrane.
The FTIR-ATR method is one of the methods of analyzing the external membrane surface.
