2008 Volume 67 Issue 6 Pages 489-495
Immunocytochemistry techniques enable visualization of the localization of certain proteins or peptides based on antigen-antibody reaction. The techniques involve various methods of fixation, embedding, preparation of sections, and staining from the light-microscopic level to the electron-microscopic level. We use the ABC method and the immunofluorescence method for analysis at the light-microscopic level, and the post embedding immunogold method for analysis at the electron-microscopic level. Here, we describe the distribution of substance-P and calcitonin gene-related peptide in the vestibular endorgans as detected by the ABC method and immunofluorescence double-labeling, as well as the subcellular localization of glutamate, glutamate transporter GLAST, and AMPA-type glutamate transporter GluR detected by the post embedding immunogold method. Through these findings, we demonstrate the usefulness of these techniques with which we have obtained satisfying results.
