Abstract
Biotic ligand model（BLM）was modified to predict metal mixture effect on algae based on the assumption that toxic mode of action is same among metals. Algal toxicity test of Zn, Cu, and Cd using green alga Pseudokirchneriella subcapitata was conducted, and the result was used for deriving BLM parameters. In addition, metal mixture algal toxicity test was conducted under the combination of Zn-Cu, Zn-Cd, and Cu-Cd. The experimental data was compared to prediction by mixture BLM. Furthermore, existing predictive models of mixture effect, concentration additive model and independent action model, were also used for predict metal mixture effect. Independent action model was the best predictor, suggesting that the toxic mode of action green algae differ among these metals.
