Abstract
Toxic chemicals released into the aquatic environment do not exist independently but coexist and they have complex and interactive effects on aquatic organisms. In some cases, it is considered that they have synergistic effects. Therefore it is very important to detect quickly and easily interactive effects of toxic chemicals in order to preserve the environment from chemical pollution. In this study, interactive effects among heavy metals and agricultural chemicals, Imidacloprid, were investigated using Daphnia magna. The data were analyzed by the method of Isobologram, where a traditional swimming inhibition test and a mobility analysis test proposed by the authors were used. The experimental results were as follows; the interactive effect of lead (Pb2+) vs. Cadmium (Cd2+) was synergistic, Cd2+ vs. Zinc (Zn2+) and Pb2+ vs. Zn2+ were antagonistic, Imidacoprid vs. Pb2+ was antagonistic and Imidacloprid vs. Zn2+ was additive. We could get a high correlation between a traditional swimming inhibition test and a new mobility inhibition test using D.magna.
