Abstract
We studied the applicability of our colorimetric monosaccharide assay based on the direct molybdosilicate reduction (DMoR) to evaluate saccharification reaction to produces glucose. DMoR method is simpler than conventional method which used chromatographic or enzymatic reaction method. The method comprises mixing a molybdosilicate solution with sample, and heating the mixture, in which the yellow molybdosilicate species is reduced directly by the monosaccharide to blue heteroplyanion. Thus, glucose gave the color change of the mixture depending on its concentration. On the other hand, maltose and cellobiose and starch showed no significant color change at the same concentration level. This present method was applied to determination of the glucose in the enzymatic reaction mixture of water-soluble starch and the extracts from sweet potato with glucoamylase. This present colorimetric results were good agreement with those by conventional electrochemical technique.
