A Method for Determining the Optimum Temperature for a Polyphenol Separation Process by Reversed Phase Chromatography

Abstract

A method for determining the optimum temperature for the separation of two polyphenols by polystyrene divinylbenzene resin reversed phase liquid chromatography with the ethanol-water mixture mobile phase was proposed based on the iso-resolution curve concept. The distribution coefficient and the stationary phase diffusion coefficient were formulated as a function of temperature T and ethanol concentration I. From the iso-resolution curve, the productivity P* was calculated as a function of T. It was found that there is an optimum T, where the highest P* can be obtained.