Abstract
Characteristics of the bacteria KP-991 and KP-992 strains isolated from soy sauce of which the bacterial spore was stable against heating for 10min at 100°C was investigated. The isolated bacteria were identified to be Bacillus amyloliquefaciens (Fukumoto) Priest et al. from the results of observation of morphology, physiological tests and analysis of 16S rDNA. The KP-991 and KP-992 strains were more stable against heating than the type strains of B. amyloliquefaciens, B. subtilis Cohn and B. megaterium de Bary. The KP-991 and KP-992 strains survived in a trial product of dilution-free seasoning because of the high thermostability and remarkably grew in two kinds of dilution-free seasoning on the market. It is suggested that the dilution-free seasoning made from the soy sauce contaminated by the KP-991 or KP-992 strain putrefies.
