Kitchen Refuse Utilization for Succinic Acid Production by Actinobacillus succinogenes ATCC 55618

Abstract

New potential application of kitchen refuse, succinic acid fermentation by Actinobacillus succinogenes ATCC 55618 to produce raw material of poly (butylene succinate) (PBS) was studied in this work. Preparation of kitchen refuse as media for succinic acid fermentation by A. succinogenes has been established. Succinic acid production and growth of A. succinogenes using kitchen refuse as substrate were significantly affected by additional nitrogen source and external gas composition The optimal kitchen refuse medium for maximal succinic acid production was 4% (w/v) glucose and 0.2% (w/v) yeast extract, biotin which support enzyme (Phosphoenolpyruvate carboxykinase) reaction in succinic acid fermentation was not essential. At optimal media and external CO₂ supply, the highest succinic acid production was 68.3±0.7% (w/w) (based on glucose consumption) in flask reaction size with shaking rate of 65 rpm at 39°C, after 48 h. Succinic acid production with 68.5% (w/w) and 30.4 g/L of final concentration, resulted in productivity with 0.6 g/L/h were obtained when conducted in 1-L of bioreactor (Under controlled pH at 6.5 by 5 M NaOH, agitation speed of 100 rpm, a CO₂ gas sparge rate of 1.0 vvm and at 39°C) . Kitchen refuse utilization for succinic acid by A. succinogenes suggested a promising practical way to produce substrate for biodegradable plastic synthesis.