Detection and Genotyping of Flavobacterium psychrophilum by PCR targeted to Peptidyl-prolyl cis-trans isomerase C Gene

Abstract

Flavobacterium psychrophilum can be classified into two genotypes, A and B, by the polymorphism in an unexpected product of 290 bp that is amplified with universal primers for gyrase subunit B gene. We identified the 290 bp fragment as a part of coding region of peptidyl-prolyl cistrans isomerase C (PPIC) gene. Using PCR newly designed for PPIC gene, specific amplification was observed in all F. psychrophilum strains tested, and no amplification occurred in any other bacterial species tested. Moreover, genotype A was found only in isolates from ayu Plecoglossus altivelis.