Vaccination with Acetone-dry Bacterin against Bacterial Hemorragic Ascites of Ayu Plecoglossus altivelis

Abstract

We developed an acetone-dry oral bacterin against bacterial hemorragic ascites of ayu Plecoglossus altivelis. The bacterin was prepared from the cultured cells of Pseudomonas plecoglossicida, which were killed in aceton at 37°C for 2 h and dehydrated. The experiment was performed twice in 2002. The bacterin was orally administered to ayu (average body weight: 4.0 g and 3.6 g) with food twice at 2-week-interval. The fish were challenged by immersion or injection with P. plecoglossicida 2 weeks after the second vaccination. The bacterin showed significant efficacy with RPS of 40-79 %.