Study of Adherence, Invasion and Survival of Hafnia alvei in RTG-2

Daniel Padilla; Felix Acosta; Judit Vega; Lita Sorroza; Lorena Román; Jimena Bravo; Fernando Real; José Vivas

doi:10.3147/jsfp.45.179

Short Communications

Study of Adherence, Invasion and Survival of Hafnia alvei in RTG-2

Daniel Padilla, Felix Acosta, Judit Vega, Lita Sorroza, Lorena Román, Jimena Bravo, Fernando Real, José Vivas

Author information

Daniel Padilla
University Institute of Animal Health (IUSA), University of Las Palmas de Gran Canaria
Felix Acosta
University Institute of Animal Health (IUSA), University of Las Palmas de Gran Canaria
Judit Vega
University Institute of Animal Health (IUSA), University of Las Palmas de Gran Canaria
Lita Sorroza
University Institute of Animal Health (IUSA), University of Las Palmas de Gran Canaria
Lorena Román
University Institute of Animal Health (IUSA), University of Las Palmas de Gran Canaria
Jimena Bravo
University Institute of Animal Health (IUSA), University of Las Palmas de Gran Canaria
Fernando Real
University Institute of Animal Health (IUSA), University of Las Palmas de Gran Canaria
José Vivas
Santa Cruz de Liencres Hospital, Marqués de Valdecilla-IFIMAV Foundation, Department of Microbiology

Keywords: Hafnia alvei, adherence, invasion, RTG-2

JOURNAL FREE ACCESS

2010 Volume 45 Issue 4 Pages 179-182

DOI https://doi.org/10.3147/jsfp.45.179

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Abstract

Hafnia alvei is a Gram-negative bacillus that belongs to the family Enterobacteriaceae and has been isolated from different fish species. H. alvei causes a wide variety of diseases, including hemorrhagic septicemia, ikidney ilesions and mortality in different fish species, as rainbow trout Oncorhynchus mykiss, cherry salmon O. masou, and brown trout Salmo trutta. The aim of this study is to investigate the interaction of different H. alvei strains with RTG-2 by gentamicin protection and immunofluorescence assays. Our results demonstrated a considerable potential of H. alvei to attach to RTG-2 cells, and that the internalized strains remained viable in the cells for 48 h.

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