Multiple Drug-resistant Strains of Aeromonas hydrophila Isolated from Tilapia Farms in Thailand

Nutcharnart Tipmongkolsilp; Carmelo S. del Castillo; Jun-ichi Hikima; Tae-Sung Jung; Hidehiro Kondo; Ikuo Hirono; Takashi Aoki

doi:10.3147/jsfp.47.56

Research Articles

Multiple Drug-resistant Strains of Aeromonas hydrophila Isolated from Tilapia Farms in Thailand

Nutcharnart Tipmongkolsilp, Carmelo S. del Castillo, Jun-ichi Hikima, Tae-Sung Jung, Hidehiro Kondo, Ikuo Hirono, Takashi Aoki

Author information

Nutcharnart Tipmongkolsilp
Laboratory of Genome Science, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology
Carmelo S. del Castillo
Aquatic Biotechnology Center, College of Veterinary Medicine, Gyeongsang National University
Jun-ichi Hikima
Aquatic Biotechnology Center, College of Veterinary Medicine, Gyeongsang National University
Tae-Sung Jung
Aquatic Biotechnology Center, College of Veterinary Medicine, Gyeongsang National University
Hidehiro Kondo
Laboratory of Genome Science, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology
Ikuo Hirono
Laboratory of Genome Science, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology
Takashi Aoki
Laboratory of Genome Science, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology
Aquatic Biotechnology Center, College of Veterinary Medicine, Gyeongsang National University

Keywords: Aeromonas hydrophila, antimicrobial resistance, transferrable R-plasmid, Oreochromis niloticus, tilapia

JOURNAL FREE ACCESS

2012 Volume 47 Issue 2 Pages 56-63

DOI https://doi.org/10.3147/jsfp.47.56

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Abstract

Fifty-five Aeromonas hydrophila isolates, which were collected from infected tilapia Oreochromis niloticus at aquaculture farms in Thailand, were tested for their antimicrobial susceptibilities against 11 drugs including ampicillin (ABPC), kanamycin (KM), streptomycin (SM), chloramphenicol (CP), florfenicol (FF), tetracycline (TC), sulfamonomethoxine (SMMX), trimethoprim (TMP) and three quinolones (QNs). All strains showed resistance to one or more drugs except FF, and more than half of the strains showed resistance to five drugs and more. These isolates were conjugated with Escherichia coli for detection of the transferable R-plasmid, and only one transferable R-plasmid, which encoded resistance to the combination of ABPC, CP, SM, SMMX and TC, was found from one isolate. This R-plasmid contained blaOXA-35 (ABPC resistance), cat2 (CP resistance), aadA1 (SM resistance), sul1 (SMMX resistance) and tetA (TC resistance).

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