Abstract
We optimized the quantitative detection of red seabream iridovirus (RSIV) genome from splenic tissues of rock bream Oplegnathus fasciatus using the quantitative polymerase chain reaction assay targeting the Pst I fragment gene. The regression line for quantitative detection of the RSIV genome was y = -0.266x + 10.95 (amplification efficacy: 84.5%), which was available between Ct 8 and 33. The quantitative-detection limit of the assay was 103.5 genomes/mg. The RSIV genome was under the quantitative-detection limit in fish by day 7 of RSIV inoculation, whereas ≥ 106.71±1.77 genomes/mg (mean ± SD) of RSIV were detected in fish from 10 days after RSIV inoculation.
