Fish Pathology
Online ISSN : 1881-7335
Print ISSN : 0388-788X
ISSN-L : 0388-788X
Proceedings of 9th Symposium on Diseases in Asian Aquaculture
A Novel Method for Detection of Fish Iridovirus in Groupers using Nano-Magnetic Particles
Ming Wei LuHsiang Ping KuoHsin Yiu ChouEe Ying LingChia Ling ChungYu Fang HungHerng Er HorngJen Leih WuGuor Mour HerZwe Ling Kong
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JOURNALS OPEN ACCESS

2016 Volume 51 Issue Special-issue Pages S54-S59

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Abstract

Iridovirus is a serious aquatic virus infecting variable species of fish and frog. Development of a rapid, reliable detection method is an important issue for commercial and scientific purposes. With specific anti-body-functionalized magnetic nanoparticles, viruses can be bound immunologically and quantitatively detected by measuring the related magnetic signals. Immunomagnetic reduction (IMR) is a technique to measure the magnetic signals change in this work. Using this assay, the low-detection limit of iridovirus was found 101 TCID50/mL. There was no significant interference from the other grouper viruses including nervous necrosis virus (NNV). The iridovirus concentration in grouper detected with IMR was 91% related to real-time polymerase chain reaction (real-time PCR). These results demonstrated the feasibility of IMR on iridovirus screening in grouper.

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© 2016 The Japanese Society of Fish Pathology
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