A Novel Method for Detection of Fish Iridovirus in Groupers using Nano-Magnetic Particles

Ming Wei Lu; Hsiang Ping Kuo; Hsin Yiu Chou; Ee Ying Ling; Chia Ling Chung; Yu Fang Hung; Herng Er Horng; Jen Leih Wu; Guor Mour Her; Zwe Ling Kong

doi:10.3147/jsfp.51.S54

Proceedings of 9th Symposium on Diseases in Asian Aquaculture

A Novel Method for Detection of Fish Iridovirus in Groupers using Nano-Magnetic Particles

Ming Wei Lu, Hsiang Ping Kuo, Hsin Yiu Chou, Ee Ying Ling, Chia Ling Chung, Yu Fang Hung, Herng Er Horng, Jen Leih Wu, Guor Mour Her, Zwe Ling Kong

Author information

Ming Wei Lu
Department of Aquaculture, National Taiwan Ocean University
National Taiwan Ocean University Center of Excellence for the Oceans, National Taiwan Ocean University
Hsiang Ping Kuo
Department of Food Science, National Taiwan Ocean University
Hsin Yiu Chou
Department of Aquaculture, National Taiwan Ocean University
National Taiwan Ocean University Center of Excellence for the Oceans, National Taiwan Ocean University
Ee Ying Ling
Department of Aquaculture, National Taiwan Ocean University
Chia Ling Chung
Department of Aquaculture, National Taiwan Ocean University
Yu Fang Hung
Department of Aquaculture, National Taiwan Ocean University
Herng Er Horng
Institute of Electro-optical Science and Technology, National Taiwan Normal University
Jen Leih Wu
Institute of Cellular and Organism Biology, Academia Sinica
Guor Mour Her
Department of Bioscience and Biotechnology, National Taiwan Ocean University
Zwe Ling Kong
Department of Food Science, National Taiwan Ocean University

Keywords: iridovirus, magnetic nanoparticles, immunomagnetic reduction, real-time polymerase chain reaction

JOURNAL OPEN ACCESS

2016 Volume 51 Issue Special-issue Pages S54-S59

DOI https://doi.org/10.3147/jsfp.51.S54

Details

Abstract

Iridovirus is a serious aquatic virus infecting variable species of fish and frog. Development of a rapid, reliable detection method is an important issue for commercial and scientific purposes. With specific anti-body-functionalized magnetic nanoparticles, viruses can be bound immunologically and quantitatively detected by measuring the related magnetic signals. Immunomagnetic reduction (IMR) is a technique to measure the magnetic signals change in this work. Using this assay, the low-detection limit of iridovirus was found 10¹ TCID₅₀/mL. There was no significant interference from the other grouper viruses including nervous necrosis virus (NNV). The iridovirus concentration in grouper detected with IMR was 91% related to real-time polymerase chain reaction (real-time PCR). These results demonstrated the feasibility of IMR on iridovirus screening in grouper.

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