Fish Pathology
Online ISSN : 1881-7335
Print ISSN : 0388-788X
ISSN-L : 0388-788X
Short Communications
Development of Anti-atypical Aeromonas salmonicida Monoclonal Antibodies for Diagnosis of “New Ulcer Disease” in Koi Carp
Tomonori SomamotoShinpei MaruyamaTakahiro NagasawaMiki NakaoAtsushi SatoHajime HattaMitsuru SatoYukie Murakami-YamaguchiKumiko Kizu-MoriYuki HirakawaHiroshi Narita
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2018 Volume 53 Issue 1 Pages 36-39

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Abstract

To develop an accurate diagnosis of “new ulcer disease” in koi carp, we produced four monoclonal antibodies (mAbs) against a strain of atypical Aeromonas salmonicida isolated from koi carp. These mAbs did not cross-react with an isolate of atypical A. salmonicida from Japanese flounder and other pathogenic bacteria. Re-isolation from artificially infected koi carp was achieved by selecting the blue colonies on agar medium containing Coomassie brilliant blue, and some of the colonies were detected by immunofluorescent staining using the mAbs. These results suggested that the mAbs can distinguish atypical A. salmonicida from koi carp from resident aeromonads.

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© 2018 The Japanese Society of Fish Pathology
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