Studies on the Pathogenic Fungus Associated with Black Gill Disease of Kuruma Prawn, Penaeus japonicus-II

Some of the Note on the BG-Fusarium

Abstract

Results of experiments on the effect of temperature on the survival of conidia of BG-Fusarium in sterile sea water and sterile distilled water suggested that the organism can survive in each water for prolonged of time. The survival rate was about 80% even after 140 days in each water at 10 to 30°C. Conidia of BG-Fusarium in sterile sea water, however, failed to survive 140 and 105 days when they were held at 5°C and 37°C, respectively. On the contrary those in sterile distilled water failed to survive 70 and 21 days when they were held at 5°C and 37°C, respectively.
The BG-Fusarium grew well on Sabouraud dextrose agar containing 0 to 0.5% NaCl, and did up to 10% NaCl, but failed to grow at 12% NaCl. It grew in Sabouraud dextrose broth at the pH range from 4 to 11.
All the BG-Fusarium strains and F. solani F-21 (plant pathogen) were resistant to cycloheximide. Two strains, F-22 and F-23 of F. solani, however, were susceptibile to the antibiotic. Malachite green completely inhibited mycelial growth at 6.3 ppm. Sodium dichloroisocyanurate was found to kill the conidia of BG-Fusarium at a concentration of 6.2 ppm as active ingredient.