Studies on the Pathogenic Fungus of Mycotic Granulomatosis—III.

Development of the Medium for MG-fungus

Abstract

MG-fungus, causative agent of mycotic granulomatosis incubated at 25°C on the FME agar containing 10% fish meat extract added various amounts of glucose showed the best growth on the FME agar containing 1% glucose.
Among several media tested a medium which consists of 1% glucose, 0.25% yeast extract and 1.5% agar was the best for the growth of MG-fungus. This medium was named as GY-medium. The fungus grew better on the GY-medium than on the FME agar, though it grew somewhat better on a modified FME agar containing 1% glucose than on the GY-medium. The fungus was capable of growing on an agar containing 0.25% yeast extract only, but incapable on an agar with 1% glucose only. This showed that a growth factor of the fungus existed in yeast extract.
The fungus grows on neither Sabouraud dextrose agar nor Czapex dox agar. Culturing tests using the FME agar added in various concentrations the main ingredients of Sabouraud dextrose agar and those of Czapex dox agar revealed that a combination of 4% glucose and 1% peptone and that of 3% glucose and 0.1% K₂HPO₄ inhibit the growth of the fungus.