Fish Pathology
Online ISSN : 1881-7335
Print ISSN : 0388-788X
ISSN-L : 0388-788X
Monolayer Cell Cultures from Marine Fishes
Yoshiichiro WATANABEHiroshi HANADAMunehiro USHIYAMA
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JOURNAL FREE ACCESS

1981 Volume 15 Issue 3-4 Pages 201-205

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Abstract
Cell lines were begun from kidney tissues of yellow tail (Seriola quinqueradiata) and sea bass (Lateolabrax japonicus), and from the fry of red sea bream (Chrysophrys major). The cells are grown in MEM-20 enriched with NaCl, incubated at 20°C and subsequent transfers maintained.
Cell lines derived from the kidney of yellow tail, designated YTK, have been subcultured 64 times since its initiation on July 13, 1977, from the fry of red sea bream, designated RSBF, have been subcultured 28 times since its initiation on May 19, 1978, and from the kidney of sea bass, designated SBK, have been subcultured 31 times since its initiation on October 11, 1978.
The cells were tested for growth curve and sensitivity to the freshwater fish viruses IPNV and IHNV.
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© The Japanese Society of Fish Pathology
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