1982 Volume 17 Issue 2 Pages 115-118
Virological examination was carried out on the kidney and spleen of anadromous amago caught by the floating gill net in Nagara River and Miyagawa River in May, 1981. Tissue extracts were inoculated onto CHSE-214(chinook salmon embryo)cells.
Virus which exhibited cytopathic effect in CHSE-214 cells were isolated from 9 of 71 fish from Nagara River and 1 of 4 fish from Miyagawa River. The isolates both from Nagara River and Miyagawa River were neutralized by anti-IPNV(Gifu)rabbit serum, indicated that they were antigenically related to IPN virus.
Among 71 sera of fish from Nagara River, 20 specimens showed the neutralization titer over 1:8 against IPNV.
The source of virus was discussed in relation to the released stock cultured in pond.