Abstract
Precipitating antibody against crude lipopolysaccharide (LPS) of Pasteurella piscicida in the serum of immature yellowtail, Seriola quinqueradiata, immunized with the cellular antigen was demonstrated by gel diffusion method (Fig.1).The serum from immunized yellowtail showed a precipitin line formation when the specific soluble antigen, LPS was reacted against the fish serum after electrophoresis. The single line occurred in the μ-globulin region identified to yellowtail immunoglobulin (Fig. 2).The precipitating antibody activity was detected in the high molecular weight fraction of gel filtration on Sepharose 6B (Fig.3).
It is concluded that precipitating antibody produced in the serum of immature yellowtail corresponds with macroglobulin in young fish.
