1985 Volume 20 Issue 2-3 Pages 435-443
Infectious hematopoietic necrosis virus (IHNV) was formalin inactivated and assayed for immunogenicity in rainbow trout (Salmo gairdneri) delivering the immunogen by intraperitoneal (IP) injection or immersion then challenging with virulent virus by IP injection or waterborne route. Concentrated virus incubated with 0.2% formalin for 6 days at 4°C was found to be inactivated with a high immunogenicity. Using this preparation, IP delivered immunogen showed protection one week after delivery (5.2°C) and persisted for at least 56 days at 5.4°C and 70 days at 11.5°C. A medium to maintain immunogenicity after storage was not found. The IHNV immediately after inactivation was the most effective immunogen. Inactivated IHNV delivered by immersion showed significant immunity as compared to non-immunized trout after waterborne challenge.
