Abstract
Changes in bilirubin contents and composition in the bile of starved and CCl4 injected carp Cyprinus carpio were examined by diazo methods. The doses of CCl4 injection were 10, 40, and 100μl/100g body weight.
In the bile of control carp, total, conjugated, and unconjugated bilirubin contens were 0.03-0.13, 0.02-0.08, and 0.01-0.02 mg /ml, respectively. During the starvation, total and conjugated bilirubin contents gradually increased but unconjugated bilirubin contents did not change. After 30 days, total and conjugated bilirubin contents were 22-26 times higher than those of control.
In the bile of CCl4 injected carp, total and conjugated bilirubin contents increased rapidly. Both contents were 8-18 times higher than those of control one day after injection. Unconjugated bilirubin contents in carps injected with 40μl and 100μl CCl4 were increased 5 days and 2 days after injection, respectively.
