Abstract
The enzymatic properties of bacteriolytic substances in the homogenates of skin mucus and intestine of carp (Cyprinus carpio) was examined. The bacteriolytic activity was estimated by the turbidimetric method using acetone-ether-dried cells of Micrococcus lysodeikticus as the substrate.
The skin mucus extracted with 0.001M phosphate buffer of pH 6.5 appeared the maximal bacteriolytic activity at 20°C. And intestine homogenized with 0.001M phosphate buffer of pH 6.0 showed the maximal activity at 40°C. The bacteriolytic activity of both extractions from the skin mucus and the intestine with the acidic buffer (pH 4.0) were relatively stable against to heating of 100°C for 10 minutes but those in neutral(pH 7.0) or alkaline (pH 9.0) buffer were liable to be inactivated.
