Detection of Renibacterium salmoninarum by a Modified Peroxidase-antiperoxidase (PAP) Procedure

Abstract

The peroxidase-antiperoxidase (PAP) procedure, modified enzyme immunoassay was applied to the detection of Renibacterium salmoninarum. This method did not indicate any cross reactivity with other fish pathogens, and was specific to BKD. The sensitivity of this proceudre was 1 × 10³ cells/ml which was at least 10 times greater than the indirect fluorescent antibody technique. The PAP procedure was tested in a BKD survey, and gave excellent results when compared with other diagnostic procedures.