Fish Pathology
Online ISSN : 1881-7335
Print ISSN : 0388-788X
ISSN-L : 0388-788X
Changes in the Agglutinating Antibody Titer and Serum Protein Composition of Colored Carp After Immunization with Aeromonas hydrophila
Riichi KUSUDAChan-fu CHENKenji KAWAI
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1987 Volume 22 Issue 3 Pages 141-146


Fluctuation in the agglutinating antibody titer and changes in the properties of serum proteins have been investigated using gel filtration and electrophoresis during the course of the immune response of colored carp, Cyprinus carpio, against Aeromonas hydrophila. Fish were injected intraperitoneally with formalin killed A. hydrophila and reared at 21°C for five weeks. Agglutinating antibody titer rose one week after immunization and the highest titers ranging 1: 2, 048 to 8, 192 were observed four weeks after immunization. The serum of immunized carp was separated into four fractions by gel filtration with Sephadex G-200. Agglutinating activity was found only in the first fraction. Cellulose acetate membrane electrophoresis pattern did not show any difference in the protein composition between immunized and non-immunized fish sera. The agar gel immunoelectrophoresis pattern of the serum samples demonstrated that some proteins from the immunized fish shifted towards the anode in the area extending from the albumins to α-globulins. Polyacrylamide gel disc eletrophoresis showed an increase in concentration of proteins (Rm=0.10 to 0.15) which were eluted in the first fraction of gel filtration.

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