Abstract
Actual net charges of live cells (RTG-2 cells) were changeable in response to nutrients. Intermetabolites of glycolytic pathway and tricarboxylic acid (TCA) cycle were responsible for net positive and negative charges of RTG-2 cells, respectively. The actual net charges were determined by electrophoresis at pH 7.0 in an appropriate device at 15°C. Infectious pancreatic necrosis (IPN) virus infection shifted the actual net charges of RTG-2 cells from neutral to positive during electrophoresis with minimum essential medium (MEM). Uninfectecd RTG-2 cells used both glucose and amino acids of MEM for nicotinamide adenine dinucleotide+ (NAD+)- and nicotinamide adenine dinucleotide phosphate+ (NADP+)-dependent dehydrogenation. IPN virus infected RTG-2 cells used glucose for the dehydrogenation, and did not used amino acids for it. The lactate dehydrogenase (LDH) activity of the virus infected cells was more enhanced than that of uninfected cells. Instead, isocitrate dehydrogenase (IDH) and malate dehydrogenase (MDH) activities were lower in the virus infected cells. The uninfected cells produced lactic acid and adenosine triphosphate (ATP) from both glucose and amino acids. The virus infected cells produced lactic acid and ATP from glucose. These results indicate that IPN virus infection induces the enhancement of LDH activity and the reduction of IDH and MDH activities to the host cells. The altered dehydrogenases produces further resultant modulation of energy metabolism through glycolysis and TCA cycle.
