Abstract
Surface membrane immunoglobulin(smlg)on yellowtail blood cells was detected by fluorescent antibody technique(FAT)using anti-yellowtail immunoglobulin M rabbit IgG.
For the detection of smIg cells, direct FAT was more convenient than the indirect method since it was relatively simple and gave less nonspecific staining. Yellowtail smIg cells were capable of cap formation at 4°C and an optimum of 18-25°C. Antigen-bound smIg disappeared within 3 to 4.5hr at 25°C, and appeared again after 6 to 24 hr in RPMI 1640 medium withoutanti-yellowtail immunoglobulin M rabbit IgG. However, it did not appear again in an excess of the anti-yellowtail immunoglobulin M rabbit IgG. Cap formation was inhibited by iodoacetamide, sodium azide, dinitrophenol and potassium cyanid. Cap formation did not occurwith monovalent antibody. The data show that the smIg-bearing cells of yellowtail are similarto mammalian B lymphocytes.
