Abstract
We are reporting the development of two monoclonal antibodies which recognize ten separate isolates of Renibacterium salmoninarum. Both monoclonal antibodies recognize a 57 kDa protein doublet and several lower molecular weight antigens present in bacterial lysates. The 57 kDa antigen appears to correlate with a 57 kDa surface protein named Antigen F by GETCHELL et al. (1985).Antibody capture ELISA analysis indicates that the antibodies recognize different epitopes of the same protein (s). Cross-reactions were not observed with control bacteria or with normal chinook salmon serum proteins.
