Abstract
Glycogen content was determined in the neutrophils of eel, Anguilla japonica. Prior to collecting neutrophils, the eel was intraperitoneally injected with formalin-killed Edwardsiella tarda, 2% casein or physiological saline as an irritant. Samples were taken from head kidney, peripheral blood and peritonel cavity exudate. Separation of neutrophils from the samples was carried out by centrifuging cell suspension loaded on a gradient Ficoll-Metrizoate solution. Neutrophils were washed with MEM supplemented with 1mg/ml of glucose, because glycogen depletion was demonstrated in the neutrophils washed with glucose-free Krebs-Ringer phosphate.
Glycogen content of neutrophils in the fish injected with 2% casein or formalin-killed E. tarda was significantly higher than that in the fish injected with physilological saline. Both the peripheral and peritoneal neutrophils contained more glycogen than head kidney neutrophils.
