Abstract
Three cell lines derived from spleen and kidney of black porgy (Acanthopagrus schlegeli), designated as BPS-1, BPS-4, and BPK, were established and their characteristics were determined. The BPS-1cell line and BPS-4 cell line consist of epithelial-like and fibroblast-like cells, respectively. The BPK cell line contains both fibroblast-like and epithelial-like cells. Using Leibovitz's-15 medium supplemented with 10%fetal calf serum and 0.15M NaCl at 28°C, BPS-1, BPS-4 and BPK cells have been passaged 105, 104, and 87 times, respectively.
The chromosome numbers of the BPS-4 cell ranged from 10 to 58 with a modal number of 30 at passage 77, while the chromosome numbers for the BPS-1 cells and the BPK cells varied even more widely from 25 to 98 at passage 76 and 9 to 94 at passage 28, respectively.With low seeding densities, all the three cell lines showed a low plating efficiency.All three cell lines were susceptible to a variety of fish birnaviruses and herpesviruses from eels in Taiwan.
