Abstract
Virucidal effects of invert soaps on IPNV and IHNV were tested.
Three kinds of invert soap formulae were used : two formulae contained benzalkonium chloride and the other contained benzethonium chloride in the ingredient. All formulae inactivated IHNV by the exposure to 0.08% solutions for 15 seconds, but did not inactivate IPNV. Addition of 10% fetal calf serum to IHNV suspension reduced the virucidal activity of invert soaps : a fetal calf serum decreased the virucidal activities of benzalkonium chloride and benzethonium chloride to 1/8-1/4 and 1/16, respectively.