Abstract
Growth responses to three different media, temperatures, and sodium chloride concentrations in the media were determined for 13 salmonid and 14 non-salmonid fish cell lines. Most cell lines showed better growth in Eagle's MEM than in Medium 199 or in Leivobitz L-15 medium. Nine salmonid cell lines grew well in the normal sodium bicarbonate buffer in Eagle's MEM, while 11 non-salmonid cell lines grew better in Eagle's MEM buffered with either HEPES-bicarbonate or Tris-bicarbonate. Optimum temperature for growth ranged from 15 to 20°C for almost all salmonid cells and 20 to 30°C for non-salmonid cells. Most of the cell lines showed highest growth in commercial medium preparations with the lowest concentration of sodium chloride (0.116M) examined. However, three of the six cell lines derived from rainbow trout, Oncorhynchus mykiss and cell lines from eels, Anguilla japonica, showed optimum growth response at a higher sodium chloride concentration of 0.171M in the medium.
