1994 Volume 29 Issue 2 Pages 113-120
A new rapid identification method of V. anguillarum from cultured fish was developed. A new selective and differential medium (V-1 medium) for Vibrio species was subjected to the primary isolation of Vibrio spp. from diseased cultured red seabream and yellowtail. Fifty strains, which were tentatively identified as the genus Vibrio, were examined on their species' levels by DNA homology values obtained from a quantitative colorimetric microdillution plate hybridization method. The identification of V. anguillarum was made when the homology values with type strain were higher than 75%. By using this method, 6 out of 50 strains of Vibrio spp. were identified as V. anguillarum. It was possible to identify V. anguillarum within 4 days from the primary isolation by this method consisting of selective isolation and DNA-DNA hybridization.
