Abstract
To clarify the production kinetics of antigenicity of virion polypeptides of yellowtail ascites virus (YAV), time course of the production of capsid proteins, VP2 and VP3, in infected CHSE-214 cells was surveyed by western blotting using polyclonal antisera against purified virion, VP2 or VP3. Pre-VP2 and VP3 were detected at 3h after infection, whereas matured VP2 appeared after 7h. The virus titer in the culture supernatant increased at 10h after infection. These results suggest that the cleavage of polyprotein to pre-VP2 and VP3 occurs in early period of infection and the VP2 maturation occurs after eclipse stage.In serological analysis, anti-VP3 serum did not neutralized the virus. Reactivity of anti-VP3 serum in ELISA increased when virion was treated with SDS.
