Abstract
In vitro effects of heat extracts from firefly squid, Watasenia scintillans, (FSE-1) and desalted FSE-1 (DFSE-1) on the activities of phagocytes and lymphocyte proliferation of rainbow trout were evaluated.
In the first experiment, the effects of FSE-1 and DFSE-1 were compared by measuring respiratory burst and potential killing activities of blood phagocytes, and by measuring lymphoblastic transformation level with or without Con A. In this experiment, DFSE-1 was found more effective than FSE-1.
In the second experiment, effects of different doses of DFSE-1 were examined by measuring nitro blue tetrazorium (NBT) reduction level, respiratory burst activity and potential killing activity of head kidney macrophages and lymphocyte proliferation test with Con A or LPS. When diluted (×1-×8) DFSE-1 was added, the head kidney macrophages showed significant (p<0.05) elevation of NBT reduction, respiratory burst and potential killing activities compared to the control. The lymphoblastic transformation rate was significantly (p<0.05) higher than that of control when diluted (×1-×16) DFSE-1 was added with Con A or LPS. These results suggest that DFSE-1 administration effectively stimulate the defense mechanism of rainbow trout.
