Abstract
Three batches of Penaeus monodon (total of 66 shrimp) were tested for the presence of WSBV by 2-step PCR, in situ hybridization and dot blot analysis using a nucleic acid probe. The results of in situ hybridization and 2-step PCR were consistent and used as the standard for comparison. Except for a low number of false negatives, results of dot blot using either DNA or tissue homogenate from the gill, pleopod, eyestalk and hemolymph paralleled the results of 2-step PCR and in situ hybridization. A quick dot blot analysis was also developed, which took only 4-4.5 h to produce results. We conclude that dot blotting using tissue homogenate from the eyestalk provides a rapid, cost effective and convenient diagnostic method for WSBV detection in the field and for screening P. monodon broodstock.
