Fish Pathology
Online ISSN : 1881-7335
Print ISSN : 0388-788X
ISSN-L : 0388-788X
Detection of PRDV from Wild Adult Kuruma Prawn
Keiichi MushiakeMisao ArimotoJun SatohKoh-ichiro Mori
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1998 Volume 33 Issue 5 Pages 503-509

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Abstract

The present study was attempted to investigate the prevalence of penaeid rod-shaped DNA virus (PRDV) in wild-caught adult kuruma prawn Penaeus japonicus using a 2-step polymerase chain reaction (PCR) as a detection method. The prawns examined were captured in 5 different areas of coastal waters in Japan from July 1996 to April 1998. Individual samples of hemolymph, stomach and gonad of these wild female and male prawns were submitted to PCR test. As a result, PRDV was detected in female (10.1%, 96/955) and male (6.7%, 21/314) prawns captured in all coastal waters, although most prawns showed no white spot in the carapace. Higher detection rates were obtained from stomach, ovary and testis than from hemolymph in female and male prawns. The prevalence of PRDV in adult prawns showed a seasonal and sex variation, i.e. the prevalence in summer (July to August) was higher than in winter (December to January) in female, and the prevalence in female was higher than that in male prawns. These results suggest that wild-captured adult kuruma prawns, especially those collected in summer, become an infection source for vertical transmission of PRDV when they are used as spawners.

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© The Japanese Society of Fish Pathology
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