Abstract
Susceptibility of 32 fish cell lines against fish nodaviruses from moribund barfin flounder (Verasper moseri, BFNNV), greasy grouper (Epinephelus tauvina, GGNNV) and striped jack (Pseudocaranx dentex, SJNNV) was investigated. Cytopathic effects were observed on SSN-1 cells inoculated with the 3 viruses at 15 or 20°C, and on SBK-2 and SK, both derived from sea bass (Lates calcarifer), inoculated with GGNNV. Viral infectivity of homogenized brain and eye of moribund barfin flounder and Japanese flounder (Paralichthys olivaceus) juveniles were 107.558.55 and 106.056.55, respectively and whole bodies of striped jack larvae were 108.80>9.80 TCID50/g.