Abstract
A simple method for quantification of sodium nifurstyrenate (NFS) in a small amount of yellowtail serum was developed by use of high performance liquid chromatography. NFS was extracted with acetonitrile from serum added with NFS in vitro, injected directly into a C18 column and detected at 420 nm wavelength with 50 mM potassium phtalate (pH4) -acetonitrile (40 : 60) as a mobile phase. This method gave the average recoveries of 102% and linear relation between peak area and NFS amount. It was possible to determine the concentration of NFS from 2.5 ng to 1000 ng in 100μL serum.
