Abstract
The effect of low incubation temperature on the embryonic development and larval hatching of the monogenean Diplectanum aequans, a gill parasite of sea bass Dicentrarchus Iabrax, was studied. Groups of eggs of D. aequans were incubated at 5°C for different time periods (from 24 h to 28 days) before incubation at room temperature (18°C). Percentages of hatched larvae, aborted larvae and undeveloped embryos were estimated in comparison with the control group. Results show that eggs of the parasite are able to survive at 5°C, with a percentage that decreases as the incubation time at 5°C increases, reducing the rate of larval hatching.