Fish Pathology
Online ISSN : 1881-7335
Print ISSN : 0388-788X
ISSN-L : 0388-788X
A Rapid and Simple Assay to Determine the Proliferation of Larval and Juvehile Fish Splenocytes
Motoaki YagiKouichi HatasakiNoboru NakaharaKenji HaraKatsuyasu TachibanaMutsuyosi Tsuchimoto
Author information
JOURNAL FREE ACCESS

2001 Volume 36 Issue 2 Pages 96-98

Details
Abstract

A simple assay to determine the proliferation of larval and juvehile fish splenocytes using Alamar Blue (AB) was studied. Splenocytes were separated by Percoll gradient from Japanese flounderParalichthys olivaceus (1-year-old), then the proliferation of splenocytes stimulated by mitogen (concanavalin A : ConA, pokeweed mitogen : PWM, or lipopolysaccharide : LPS) was detected using AB. The relationship between number of splenocytes and specific absorbance exhibited a positive significant correlation. The optimum condition of this assay was 5×105 cells/well of splenocytes with mitogens (ConA 100μg/mL, PWM 10μg/mL or LPS 1μg/mL) for 72 h. Proliferation of each splenocyte from juveniles of Japanese flounder and larvae of Japanese parrotfish Ophegnathus fasciatus and tiger puffer Takifugu rubripes was detectable by this assay.

Content from these authors
© The Japanese Society of Fish Pathology
Previous article Next article
feedback
Top