Abstract
In orderto develop a vaccine for bacterial hemorrhagic ascites caused by Pseudomonas plecoglossicida in cultured ayu Plecoglossus altivelis, the efficacy of oiladjuvanted vaccines was examined. Formalin-killed P. plecoglossicida bacterin emulsified with an oil-adjuvant (MONTANIDE-ISA711 or MONTANIDE-ISA763A) or with saline was intraperitoneally injected into fish. Intraperitoneal injection with virulent P. plecoglossicida was carried out 22 days and 52 days after vaccination. The relative percent survivals of vaccinated fish were 17-58% without any adjuvants, 57-92% with ISA711 and 65-86% with ISA763A. The adjuvants remained in the body cavity of vaccinated fish for at least 65 days.
