Age-dependent Humoral Immune Response of Barfin Flounder against Recombinant Coat Protein of Barfin Flounder Nervous Necrosis Virus

Abstract

Age-dependent humoral immune response of barfin flounder Verasper moseri was investigated. One hundredμL of recombinant coat protein of barfin flounder nervous necrosis virus (BFNNV) adjusted to 250 μg/mL in Tris-EDTA buffer (pH 8.0) was injected intravascularly to 4, 5, 6 and 7 month-old fish. One month after injection, an enzyme-linked immunosorbent assay (ELISA) antibody titer was measured. Although no ELISA antibody titers were detected in injected or noninjected fish of 4 and 5 month-old, the antibody titers were remarkably increased (p<0.025) in 6 and 7 month-old fish. High ELISA antibody titers were kept for at least 2 months.