2004 Volume 39 Issue 4 Pages 189-196
Two cDNA libraries were prepared from Japanese flounder Paralichthys olivaceus kidney cells that had been treated with LPS or a combination of ConA and PMA. From these two libraries, we sequenced 373 and 379 clones, respectively. Of these 752 clones, 109 clones were characterized as immune-related. Among the immune-related cDNAs, the following have not been previously reported in fish : NK-lysin, perforin, complement C1 q, CD9 antigen, CD63 antigen, CD82 antigen, ISGF-3, IP-30, G-CSF, equistatin precursor, TLS-CHOP, fas ligand and CARD4. This study highlights the success of using ConA/PMA and LPS as mitogens to increase the number of immune-related genes expressed in Japanese flounder and demonstrates that different stimuli induce the expression of different immune-related genes.
