Abstract
Addition of paclobutrazol (PAC) to modified MS medium induced prptocorm-like body (PLB) formation from rhizome segment cultures of Cyrnbidium kanran Makino (terrestrial type). The combination of BA with PAC reduced the number of PLB. Application of BA induced shoot (protocorm-like pseudobulb with short leaves) without root. Single addition of 10μMBA had a significant effect on induction and increased the number of shoots. PAC in culture medium inhibited BA-induced leaf formation from rhizome cultures.
Application of GA3 to PLB cultures of epiphytic type Cymbidium (C. Hiroshima Golden Cup 'Sunny Moon') resulted rhizome formation. GA3 treatment at 100μM was effective for rhizome formation from FLE cultures. Single addition of 10μMPAC was effective for proliferation of PLB from 'Sunny Moon'.
