Abstract
Pressure can be a key control factor in the applied enzymology. High pressure technique can be introduced in many aspects of enzyme engineering; preparation of enzymes, enzymatic conversion of chemical substances, modification and alternation of enzyme functions in chemical or biochemical ways, and enzymatic (biochemical) processes including immobilized enzymes. It could be called as high pressure enzyme engineering. Among these, the utilization of pressure dependence of enzymatic reaction rates and of the pressure-induced change in the higher order structure of enzyme proteins are explained in detail, by taking some hydrolytic enzymes as the examples.
