1999 Volume 6 Issue 1 Pages 35-40
Excessive interleukin-8 (IL-8) plays an important role as a key mediator in neutrophil-dependent inflammatory disease.
The purpose of this study was to elucidate the time course of IL-8 exudation from the local inflammatory tissue to systemic circulation using a hydrochloric acid (HCl) injured lung model.
Fifty-six male Spraque-Dawley rats were mechanically ventilated after tracheostomy under ketamine and xylazine anesthesia. 1.5 ml·kg-1 of HCl with pH 1.5 was instilled through the endotracheal tube. The animals were randomly divided into 6 study groups according to the time at which they were sacrificed: before and 30 minutes, 1, 2, 3, and 4 hours after HCl injection. Two of the control groups did not receive HCl. The first control group was ventilated with room air, and the second control group with pure oxygen for 4 hours. IL-8 concentrations in the blood, broncho-alveolar lavage fluid (BALF), and lung tissue were measured by enzyme-linked immuno-sorbent assay (ELISA).
IL-8 in lung tissue and in BALE significantly increased one hour after HCl instillation, while IL-8 in the blood significantly increased at 4 hours.
The release of IL-8 into the blood was delayed for a few hours compared to the elevation of IL-8 in lung tissue and in BALF.
